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KMID : 0369820080380060357
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Jorunal of Korean Pharmaceutical Sciences
2008 Volume.38 No. 6 p.357 ~ p.363
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Transdermal Permeation Behavior of FITC-BSA using Microneedle
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Kim Yun-Tae
Oh A-Young
Lee Jun-Hee
Ahn Sik-Il
Park Jong-Hak
Lee Han-Koo
Khang Gil-Son
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Abstract
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Penetration rate of large molecule through skin is very low due to the barrier effect of stratum corneum. Novel microneedle treatment device with roll was designed for transdermal delivery of large molecular drugs such as vaccine and protein drugs. The permeation rates of FITC labelled bovine serum albumin (FITC-BSA) as a model protein were determined using modified Franz diffusion cell and hairless mouse skin which were treated by hydrogel or solution containing FITC-BSA. Fluorescent spectrophotometer was used to analyze the concentration of FITC-BSA. Microscope using fluorescent filter was used to capture the image and location of FITC-BSA in the skin. We confirmed that permeation rate of BSA was increased with the treatment by microneedle and was increased by the increasing frequency of treatment. Furthermore,
the permeation rate observed from hydrogel treated skin was significantly higher than that from solution treated skin.
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KEYWORD
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Transdermal, FITC-BSA, penetration, hydrogel
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